下表列出了生命科学领域多肽荧光标记染料的荧光特性,荧光吸收波长从325nm到743nm(从蓝色到红色),发射波长从386nm到770nm。
荧光染料 | Ex (nm) | Em (nm) |
Hydroxycoumarin | 325 | 386 |
Dansyl | 340 | 578 |
7-Amino-4-methylcoumarin | 351 | 430 |
methoxycoumarin | 360 | 410 |
Alexa fluor | 345 | 442 |
aminocoumarin | 350 | 445 |
AMC | 345 | 445 |
Dabcyl | 453 | -- |
Cy2 | 490 | 510 |
FAM | 495 | 517 |
Alexa fluor 488 | 494 | 517 |
Fluorescein FITC | 495 | 519 |
Alexa fluor 430 | 430 | 545 |
5-Carboxyfluorescein (5-FAM) | 492 | 518 |
Alexa fluor 532 | 530 | 555 |
HEX | 535 | 556 |
5-TAMRA | 542 | 568 |
Cy3 | 550 | 570 |
TRITC | 547 | 572 |
Alexa fluor 546 | 556 | 573 |
Alexa fluor 555 | 556 | 573 |
Dansyl | 340 | 578 |
R-phycoerythrin (PE) (489) | 565 | 578 |
Rhodamine Red-X | 560 | 580 |
Tamara | 565 | 580 |
Cy3 (512) | 550 | 570 |
Cy3.5 | 581 | 596 |
Rox | 575 | 602 |
Alexa fluor 568 | 578 | 603 |
Texas Red | 589 | 615 |
Alexa fluor 594 | 590 | 617 |
Alexa fluor | 621 | 639 |
Alexa fluor 633 | 650 | 668 |
Cy5 (625) | 650 | 670 |
Alexa fluor 660 | 663 | 690 |
Cy5.5 | 675 | 694 |
TruRed | 490; 675 | 695 |
Alexa fluor 680 | 679 | 702 |
Cy7 | 743 | 767 |